| Regional Biophysics Meeting 2005, March 16-20, Zreče, Slovenia | [MembBiophys] |
The work is devoted the experimental investigation of radiation and combined therapy influence on dielectric permittivity and structural changes of erythrocyte membranes in the patients with tumors (breast, lung, stomach cancer) in 3-cm range of wavelengths obtained by method of UHF-dielectrometry. The dependence of the complex dielectric permittivity and the frequency of water molecules dielectric relaxation in erythrocyte membranes in the patients with tumors and the donors were obtained in the temperature range of 1-50 °C. The temperature dependencies of the real and imaginary parts of permittivity of the erythrocyte membranes have been obtained. In contrast to dependencies for poor solvent, temperature dependencies of dielectric parameters for the erythrocyte membranes of the patients with tumors are of no monotonous character, they have a number of peculiarities in the temperature ranges of 2-6, 8-15, 15-17, 17-36, 36-42 and 42-46 0C. The structural changes of the erythrocyte membranes for the donors have been registered as changes at the temperature ranges of 2-6, 8-12, 25-36, 36-42 and 42-46 0C. The activation energy of water molecules dielectric relaxation has been calculated and dependences of static dielectric permittivity for these systems have been obtained to the "solvent". It has been shown that the structural transitions of erythrocyte membranes at 8°С and 15 °С in the patients with malignant tumors are accompanied by the change in the proportion of free and bound water in erythrocytes. It is supposed that the observed features of the dielectric parameters dependent on temperature accompanied by the change of activation energy are related to the dehydration of erythrocyte membranes of oncological patients. Authors proposed new method for estimation of the state and the efficacy of the radiation and combined treatment of cancer patients, basing on determination of the structural changes of erythrocyte membranes at various temperatures.
Email: liland@kharkov.ukrtel.net
Address: Grigoriev Institute for Medical Radiology, 82 Pushkinska Str., Kharkiv, Ukraine, 61024