| Regional Biophysics Meeting 2005, March 16-20, Zreče, Slovenia | [MembBiophys] |
One of the most challenging problems in structural biology of the 21st century will be to unravel the structure and function of membrane proteins. Among the spectroscopic methods, site-directed ESR spin labelling and fluorescence labelling are promising tools in the hands of a biophysicist. The techniques are based on site-directed mutagenesis in combination with specific labelling and provide specific information about the local environment, structure and dynamical properties of the labelled sites. In this presentation an overview will be given of the currents state of the art of site-directed labelling applications to get a detailed insight into the topology, structure and dynamics of membrane proteins.
Email: marcus.hemminga@wur.nl
Address: Laboratory of Biophysics, Wageningen University, P.O. Box 8128, 6700 ET Wageningen, The Netherlands, Office: Dreijenlaan 3, 6703 HA Wageningen