| Regional Biophysics Meeting 2005, March 16-20, Zreče, Slovenia | [CellBiophys] |
Effects of different surfaces on adhesion, proliferation and shape of V79 fibroblasts and epithelial CaCo-2 cells were studied. Surfaces were obtained by coating microscope slides by silan or fibronectin at different concentrations. Cell shapes were recorded by a CCD camera attached to an inverted microscope. To determine the adherence and proliferation of cells flexiperm wells, made of silicon were used and subdivided into eight rectangular chambers. We fixed one flexiperm to the slide with a coated surface, and another one to a simple glass for control. The cells were seeded in each chamber (0.7 cm2) and grown at 37ºC in a CO2 incubator. The number of cells was determined after 3, 24 and 48 hours of growth. The attached cells were counted by the inverted microscope (magnification 200x) using Image Tool program (4 measurements per chamber, each on 0.14 mm2 area). Concentration of cells (number of cells/chamber area) was calculated. Our results showed that silan had stronger inhibitory effects on cell adhesion and proliferation than fibronectin. On the silan surface 70% of cells were attached after 3 hours in comparison to control. Cells did not divide and their number remained the same after 24 and 48 hours. The effects of fibronectin on adhesion indicated about 90% attached cells after 3 hours, whereas proliferation was diminished for about 20%. The changes of cell shape were also observed: the cells were rounded on silan and well spread on fibronectin.
Email: alenka.cimpric@biofiz.mf.uni-lj.si
Address: University of Ljubljana, Faculty of Medicin, Institute of Biophysics, Lipičeva 2, SI-1000 Ljubljana, Slovenia